Application of retrotransposon-based Inter-SINE Amplified Polymorphism (ISAP) markers for the differentiation of common poplar genotypes

On the basis of retrotransposon-insertion polymorphisms, molecular markers were developed for identification and differentiation poplar (<i>Populus</i> spp.) genotypes. For this purpose, short interspersed nuclear elements (SINEs) in genome sequence <i>Populus tremula</i> identified assigned to different SINE-families. families with high copy number identity values, primers derived amplify Inter- SINE Amplified Polymorphisms (ISAPs) polymerase chain reaction (PCR). The resulting fragments produce genotype-specific fingerprints. This approach utilizes standard laboratory equipment is therefore easy use verification plant material. We demonstrate functionality three distinct ISAP primer combinations by comparison ten simple repeat (SSR) differentiate 23 Already using a single combination all genotypes can be clearly discriminated. Furthermore, cluster analysis based on divides clones according their genetic background into two subclusters (by bootstrap value 98). Our results usability trace progenies trees.